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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brown adipose tissue using UCP1 Recombinant antibody (83870-1-RR)

    WB analysis of mouse brown adipose using 83870-1-RR

    mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 83870-1-RR (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using UCP1 Recombinant antibody (83870-1-RR)

    IHC staining of rat brain using 83870-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using UCP1 Recombinant antibody (83870-1-RR)

    IHC staining of rat brain using 83870-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brown adipose tissue using UCP1 Recombinant antibody (83870-1-RR)

    IF Staining of mouse brown adipose using 83870-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brown adipose tissue using UCP1 Recombinant antibody (83870-1-RR)

    IF Staining of mouse brown adipose using 83870-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83870-1-RR

    Affinity and Kinetic Characterization of 83870-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83870-1-RR against Human UCP1 were performed. The affinity constant is 64.6 nM.

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UCP1 Recombinant antibody

UCP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83870-1-RR
Clone No.240782F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

UCP 1, UCP, Thermogenin, SLC25A7, 240782F3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brown adipose tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brown adipose tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83870-1-RR targets UCP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UCP1 fusion protein Ag20388 相同性解析による交差性が予測される生物種
Full Name uncoupling protein 1 (mitochondrial, proton carrier)
Calculated molecular weight 307 aa, 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC098168
Gene Symbol UCP1
Gene ID (NCBI) 7350
RRIDAB_3671456
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

Protocols

Product Specific Protocols
WB protocol for UCP1 antibody 83870-1-RRDownload protocol
IHC protocol for UCP1 antibody 83870-1-RRDownload protocol
IF protocol for UCP1 antibody 83870-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols