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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using UCP5 Polyclonal antibody (25223-1-AP)

    WB analysis of fetal human brain using 25223-1-AP (same clone as 25223-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25223-1-AP (UCP5 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25223-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using UCP5 Polyclonal antibody (25223-1-AP)

    WB analysis of PC-3 using 25223-1-AP (same clone as 25223-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25223-1-AP (UCP5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25223-1-PBS in a different storage buffer formulation.

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UCP5 Polyclonal antibody, PBS Only

UCP5 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 25223-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25223-1-PBS targets UCP5 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCP5 fusion protein Ag18661 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier, brain), member 14
Calculated molecular weight 325 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC119666
Gene Symbol UCP5
Gene ID (NCBI) 9016
RRIDAB_2879971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95258
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

UCP5, also known as BMCP1 (brain mitochondrial carrier protein-1), is a member of uncoupling proteins (UCPs) that catalyze proton leaks across the inner mitochondrial membrane, thus uncoupling fuel oxidation from ATP synthesis. UCPs are implicated in metabolic rate and adaptational thermoregulation. UCP5 is widely expressed with high abundance in brain and testis. Alternative splicing generates several isoforms of UCP5.