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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated UGDH Monoclonal antibody

UGDH Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2E1

Cat no : CL488-67360

Synonyms

GDH, UDP Glc dehydrogenase, UDP GlcDH, UDP glucose 6 dehydrogenase, UDP glucose dehydrogenase, UDPGDH, UGD, UGDH

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated UGDH Monoclonal anti (CL488-67360)

    IF Staining of HeLa using CL488-67360

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 UGDH antibody (CL488-67360, Clone: 1E2E1 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67360 targets UGDH in IF/ICC applications and shows reactivity with Human, Mouse, Rat, pig samples.

Tested Reactivity Human, Mouse, Rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UGDH fusion protein Ag29747 相同性解析による交差性が予測される生物種
Full Name UDP-glucose dehydrogenase
Calculated molecular weight 494 aa, 55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC022781
Gene symbol UGDH
Gene ID (NCBI) 7358
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 UGDH antibody CL488-67360Download protocol
Standard Protocols
Click here to view our Standard Protocols