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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ULBP2 Monoclonal antibody (67249-1-Ig)

    WB analysis using 67249-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67249-1-Ig (ULBP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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ULBP2 Monoclonal antibody

ULBP2 Monoclonal Antibody for WB, ELISA
Cat No. 67249-1-Ig
Clone No.1A9G8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

ALCAN alpha, N2DL 2, N2DL2, NKG2D ligand 2, NKG2DL2, RAET1H, UL16 binding protein 2, ULBP2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, TF-1 cells, K-562 cells, THP-1 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67249-1-Ig targets ULBP2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ULBP2 fusion protein Ag28879 相同性解析による交差性が予測される生物種
Full Name UL16 binding protein 2
Calculated molecular weight 246 aa, 27 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC034689
Gene Symbol ULBP2
Gene ID (NCBI) 80328
RRIDAB_2882526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BZM5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6) (PMID: 29568297; 30813924). ULBPs are MHC class I-related molecules (PMID: 11239445). ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface (PMID: 21224393; 24614922). ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.

Protocols

Product Specific Protocols
WB protocol for ULBP2 antibody 67249-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols