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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP (same clone as 27352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP (same clone as 27352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP (same clone as 27352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of human liver cancer using 27352-1-AP (same clone as 27352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of mouse skeletal muscle using 27352-1-AP (same clone as 27352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of mouse skeletal muscle using 27352-1-AP (same clone as 27352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of human liver cancer using 27352-1-AP (same clone as 27352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ULK1 Polyclonal antibody (27352-1-AP)

    IF Staining of HeLa using 27352-1-AP (same clone as 27352-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ULK1 antibody (27352-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

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ULK1 Polyclonal antibody, PBS Only

ULK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27352-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27352-1-PBS targets ULK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ULK1 fusion protein Ag22397 相同性解析による交差性が予測される生物種
Full Name unc-51-like kinase 1 (C. elegans)
Observed molecular weight 140-150 kDa
GenBank accession numberNM_003565
Gene Symbol ULK1
Gene ID (NCBI) 8408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75385
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318). ULK1 is a 113 kDa protein that it also can be detected a band of 150 kDa through the western blot (PMID: 32904616, PMID: 32899862, PMID: 37270528).