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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated UNC13A Monoclonal antibody

UNC13A Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3C3A9

Cat no : CL555-68483

Synonyms

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite®555-conjugated UNC13A Monoclonal antibody (CL555-68483)

    IF Staining of SH-SY5Y using CL555-68483

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 UNC13A antibody (CL555-68483, Clone: 3C3A9 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite®555-conjugated UNC13A Monoclonal antibody (CL555-68483)

    FC experiment of SH-SY5Y using CL555-68483

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated UNC13A Monoclonal antibody (CL555-68483, Clone:3C3A9)(red), or 0.4 ug CoraLite® Plus 555 Mouse IgG2a isotype control (11A1B2) (CL555-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-68483 targets UNC13A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name unc-13 homolog A (C. elegans)
Calculated molecular weight 193 kDa
Observed molecular weight193-210 kDa
GenBank accession numberNM_001080421
Gene symbol UNC13A
Gene ID (NCBI) 23025
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

Protocols

Product Specific Protocols
IF protocol for CL555 UNC13A antibody CL555-68483Download protocol
FC protocol for CL555 UNC13A antibody CL555-68483Download protocol
Standard Protocols
Click here to view our Standard Protocols