Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | SH-SY5Y cells |
68483-1-Ig
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-68483 targets UNC13A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Reactivity | human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | unc-13 homolog A (C. elegans) |
Calculated molecular weight | 193 kDa |
Observed molecular weight | 193-210 kDa |
GenBank accession number | NM_001080421 |
Gene Symbol | UNC13A |
Gene ID (NCBI) | 23025 |
RRID | AB_3673423 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UPW8 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.
Protocols
Product Specific Protocols | |
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IF protocol for CL555 UNC13A antibody CL555-68483 | Download protocol |
FC protocol for CL555 UNC13A antibody CL555-68483 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |