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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UNC13D Monoclonal antibody

UNC13D Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C5C1

Cat no : 67193-1-Ig

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UNC13D Monoclonal antibody (67193-1-Ig)

    WB analysis using 67193-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using UNC13D Monoclonal antibody (67193-1-Ig)

    WB analysis of RAW 264.7 using 67193-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using UNC13D Monoclonal antibody (67193-1-Ig)

    WB analysis of pig spleen using 67193-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using UNC13D Monoclonal antibody (67193-1-Ig)

    WB analysis of rat spleen using 67193-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using UNC13D Monoclonal antibody (67193-1-Ig)

    IHC staining of rat spleen using 67193-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 67193-1-Ig (UNC13D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using UNC13D Monoclonal antibody (67193-1-Ig)

    IHC staining of rat spleen using 67193-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 67193-1-Ig (UNC13D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inTHP-1 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, K-562 cells, Jurkat cells, HL-60 cells
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67193-1-Ig targets UNC13D in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UNC13D fusion protein Ag10497 相同性解析による交差性が予測される生物種
Full Name unc-13 homolog D (C. elegans)
Calculated molecular weight 1090 aa, 103 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC067084
Gene symbol UNC13D
Gene ID (NCBI) 201294
RRIDAB_2882488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UNC13D antibody 67193-1-IgDownload protocol
IHC protocol for UNC13D antibody 67193-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols