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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated UNC13D Monoclonal antibody

UNC13D Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C5C1

Cat no : CL488-67193

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated UNC13D Monoclonal an (CL488-67193)

    WB analysis using CL488-67193

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67193 (UNC13D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJurkat cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67193 targets UNC13D in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UNC13D fusion protein Ag10497 相同性解析による交差性が予測される生物種
Full Name unc-13 homolog D (C. elegans)
Calculated molecular weight 1090 aa, 103 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC067084
Gene symbol UNC13D
Gene ID (NCBI) 201294
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UNC13D is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 UNC13D antibody CL488-67193Download protocol
Standard Protocols
Click here to view our Standard Protocols