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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated UPF1 Monoclonal antibody

UPF1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B6B5

Cat no : CL488-66898

Synonyms

ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated UPF1 Monoclonal anti (CL488-66898)

    IF Staining of HepG2 using CL488-66898

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 UPF1 antibody (CL488-66898, Clone: 3B6B5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66898 targets UPF1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UPF1 fusion protein Ag28320 相同性解析による交差性が予測される生物種
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Calculated molecular weight123 kDa
Observed molecular weight 123-130 kDa
GenBank accession numberBC039817
Gene symbol UPF1
Gene ID (NCBI) 5976
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 UPF1 antibody CL488-66898Download protocol
FC protocol for CL Plus 488 UPF1 antibody CL488-66898Download protocol
Standard Protocols
Click here to view our Standard Protocols