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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated UPF1 Recombinant antibody

UPF1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3O15

Cat no : CL488-82791

Synonyms

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated UPF1 Recombinant ant (CL488-82791)

    IF Staining of HepG2 using CL488-82791

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 UPF1 antibody (CL488-82791, Clone: 3O15 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82791 targets UPF1 in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UPF1 fusion protein Ag29057 相同性解析による交差性が予測される生物種
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Calculated molecular weight123 kDa
Observed molecular weight 123-130 kDa
GenBank accession numberBC039817
Gene symbol UPF1
Gene ID (NCBI) 5976
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 UPF1 antibody CL488-82791Download protocol
Standard Protocols
Click here to view our Standard Protocols