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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UQCRC1 Recombinant antibody, PBS Only

UQCRC1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240507A6

Cat no : 83671-2-PBS

Synonyms

QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I, Complex III subunit 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC1 Recombinant antibody (83671-2-RR)

    WB analysis using 83671-2-RR (same clone as 83671-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83671-2-RR (UQCRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83671-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UQCRC1 Recombinant antibody (83671-2-RR)

    IHC staining of human ovary cancer using 83671-2-RR (same clone as 83671-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83671-2-RR (UQCRC1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83671-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83671-2-RR

    Affinity and Kinetic Characterization of 83671-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83671-2-RR against Human UQCRC1 were performed. The affinity constant is 0.294 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83671-2-PBS is UQCRC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UQCRC1 fusion protein Ag16322 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase core protein I
Calculated molecular weight 480 aa, 53 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC009586
Gene symbol UQCRC1
Gene ID (NCBI) 7384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.