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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UQCRC1 Recombinant antibody

UQCRC1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240507A6

Cat no : 83671-2-RR

Synonyms

QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I, Complex III subunit 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC1 Recombinant antibody (83671-2-RR)

    WB analysis using 83671-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83671-2-RR (UQCRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using UQCRC1 Recombinant antibody (83671-2-RR)

    IHC staining of human stomach cancer using 83671-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83671-2-RR (UQCRC1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 83671-2-RR

    Affinity and Kinetic Characterization of 83671-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83671-2-RR against Human UQCRC1 were performed. The affinity constant is 0.294 nM.

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Tested Applications

Positive WB detected inHeLa cells, L02 cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83671-2-RR is UQCRC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UQCRC1 fusion protein Ag16322 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase core protein I
Calculated molecular weight 480 aa, 53 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC009586
Gene symbol UQCRC1
Gene ID (NCBI) 7384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

Protocols

Product Specific Protocols
WB protocol for UQCRC1 antibody 83671-2-RRDownload protocol
IHC protocol for UQCRC1 antibody 83671-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols