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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UQCRC2 Recombinant antibody, PBS Only

UQCRC2 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240454D9

Cat no : 83667-2-PBS

Synonyms

Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC2 Recombinant antibody (83667-2-RR)

    WB analysis using 83667-2-RR (same clone as 83667-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83667-2-RR (UQCRC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using UQCRC2 Recombinant antibody (83667-2-RR)

    IHC staining of human stomach cancer using 83667-2-RR (same clone as 83667-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83667-2-RR (UQCRC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using UQCRC2 Recombinant antibody (83667-2-RR)

    FC experiment of HepG2 using 83667-2-RR (same clone as 83667-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug UQCRC2 Recombinant antibody (83667-2-RR, Clone:240454D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83667-2-RR

    Affinity and Kinetic Characterization of 83667-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83667-2-RR against Human UQCRC2 were performed. The affinity constant is 21.8 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83667-2-PBS is UQCRC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UQCRC2 fusion protein Ag6432 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000484
Gene symbol UQCRC2
Gene ID (NCBI) 7385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.