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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC2 Recombinant antibody (83667-2-RR)

    WB analysis using 83667-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83667-2-RR (UQCRC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using UQCRC2 Recombinant antibody (83667-2-RR)

    IHC staining of human stomach cancer using 83667-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83667-2-RR (UQCRC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Flow cytometry (FC) experiment of HepG2 cells using UQCRC2 Recombinant antibody (83667-2-RR)

    FC experiment of HepG2 using 83667-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug UQCRC2 Recombinant antibody (83667-2-RR, Clone:240454D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83667-2-RR

    Affinity and Kinetic Characterization of 83667-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83667-2-RR against Human UQCRC2 were performed. The affinity constant is 21.8 nM.

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UQCRC2 Recombinant antibody

UQCRC2 Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83667-2-RR
Clone No.240454D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83667-2-RR targets UQCRC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UQCRC2 fusion protein Ag6432 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000484
Gene Symbol UQCRC2
Gene ID (NCBI) 7385
RRIDAB_3671273
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP22695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.

Protocols

Product Specific Protocols
WB protocol for UQCRC2 antibody 83667-2-RRDownload protocol
IHC protocol for UQCRC2 antibody 83667-2-RRDownload protocol
FC protocol for UQCRC2 antibody 83667-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols