Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HEK-293 cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
68529-1-Ig targets UQCRFS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | UQCRFS1 fusion protein Ag13307 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC010035 |
| Gene Symbol | UQCRFS1 |
| Gene ID (NCBI) | 7386 |
| RRID | AB_3085237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P47985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UQCRFS1 antibody 68529-1-Ig | Download protocol |
| IF protocol for UQCRFS1 antibody 68529-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Cancerous Conditions Accelerate the Aging of Skeletal Muscle via Mitochondrial DNA Damage |