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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRFS1 Monoclonal antibody (68529-1-Ig)

    WB analysis using 68529-1-Ig (same clone as 68529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68529-1-Ig (UQCRFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68529-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UQCRFS1 Monoclonal antibody (68529-1-Ig)

    IF Staining of HeLa using 68529-1-Ig (same clone as 68529-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UQCRFS1 antibody (68529-1-Ig, Clone: 1F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68529-1-PBS in a different storage buffer formulation.

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UQCRFS1 Monoclonal antibody, PBS Only

UQCRFS1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68529-1-PBS
Clone No.1F11C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Cytochrome b-c1 complex subunit Rieske, mitochondrial, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit 5, Complex III subunit IX, Complex III subunit 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68529-1-PBS targets UQCRFS1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UQCRFS1 fusion protein Ag13307 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
Calculated molecular weight 30 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC010035
Gene Symbol UQCRFS1
Gene ID (NCBI) 7386
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP47985
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.