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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using URAT1 Recombinant antibody (82964-1-RR)

    WB analysis using 82964-1-RR (same clone as 82964-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82964-1-RR (URAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82964-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using URAT1 Recombinant antibody (82964-1-RR)

    FC experiment of HepG2 using 82964-1-RR (same clone as 82964-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug URAT1 Recombinant antibody (82964-1-RR, Clone:230204B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82964-1-PBS in a different storage buffer formulation.

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URAT1 Recombinant antibody, PBS Only

URAT1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82964-1-PBS
Clone No.230204B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82964-1-PBS targets URAT1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen URAT1 fusion protein Ag27805 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22 (organic anion/urate transporter), member 12
Calculated molecular weight 60 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC053348
Gene Symbol URAT1
Gene ID (NCBI) 116085
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96S37
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.