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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using URAT1 Recombinant antibody (82964-1-RR)

    WB analysis using 82964-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82964-1-RR (URAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using URAT1 Recombinant antibody (82964-1-RR)

    FC experiment of HepG2 using 82964-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug URAT1 Recombinant antibody (82964-1-RR, Clone:230204B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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URAT1 Recombinant antibody

URAT1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82964-1-RR
Clone No.230204B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 750
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue, human kidney tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82964-1-RR targets URAT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen URAT1 fusion protein Ag27805 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22 (organic anion/urate transporter), member 12
Calculated molecular weight 60 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC053348
Gene Symbol URAT1
Gene ID (NCBI) 116085
RRIDAB_3670710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96S37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.

Protocols

Product Specific Protocols
WB protocol for URAT1 antibody 82964-1-RRDownload protocol
FC protocol for URAT1 antibody 82964-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols