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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated URAT1 Recombinant an (CL750-82964)

    WB analysis using CL750-82964

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82964 (URAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 750-conjugated URAT1 Recombinant an (CL750-82964)

    FC experiment of HepG2 using CL750-82964

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated URAT1 Recombinant antibody (CL750-82964, Clone:230204B3)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 750-conjugated URAT1 Recombinant antibody

URAT1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82964
Clone No.230204B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Synonyms

SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, mouse liver tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82964 targets URAT1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen URAT1 fusion protein Ag27805 相同性解析による交差性が予測される生物種
Full Name solute carrier family 22 (organic anion/urate transporter), member 12
Calculated molecular weight 60 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC053348
Gene Symbol URAT1
Gene ID (NCBI) 116085
RRIDAB_3673754
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96S37
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 URAT1 antibody CL750-82964Download protocol
FC protocol for CL Plus 750 URAT1 antibody CL750-82964Download protocol
Standard Protocols
Click here to view our Standard Protocols