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  • Featured Product
  • KD/KO Validated

USP1 Monoclonal antibody

USP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C10H6

Cat no : 66069-1-Ig

Synonyms

Deubiquitinating enzyme 1, hUBP, ubiquitin specific peptidase 1, Ubiquitin thiolesterase 1, UBP, USP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using USP1 Monoclonal antibody (66069-1-Ig)

    WB analysis of HepG2 using 66069-1-Ig

    WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells.

  • Western Blot (WB) analysis of HepG2 cells using USP1 Monoclonal antibody (66069-1-Ig)

    WB analysis of HepG2 using 66069-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using USP1 Monoclonal antibody (66069-1-Ig)

    WB analysis of HeLa using 66069-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using USP1 Monoclonal antibody (66069-1-Ig)

    IHC staining of human cervical cancer using 66069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using USP1 Monoclonal antibody (66069-1-Ig)

    IHC staining of human cervical cancer using 66069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using USP1 Monoclonal antibody (66069-1-Ig)

    IHC staining of human cervical cancer using 66069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using USP1 Monoclonal antibody (66069-1-Ig)

    IHC staining of human cervical cancer using 66069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66069-1-Ig targets USP1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, monkey
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP1 fusion protein Ag5904 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 1
Calculated molecular weight 88 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC050525
Gene symbol USP1
Gene ID (NCBI) 7398
RRIDAB_11043340
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).

Protocols

Product Specific Protocols
WB protocol for USP1 antibody 66069-1-IgDownload protocol
IHC protocol for USP1 antibody 66069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkeyWB

J Virol

Ubiquitin-specific proteinase 1 stabilizes PRRSV nonstructural protein Nsp1β to promote viral replication by regulating K48 ubiquitination

Authors - Yunyun Zhai
  • KD Validated
View Article