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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using USP1 Monoclonal antibody (66069-1-Ig)

    WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using USP1 Monoclonal antibody (66069-1-Ig)

    WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using USP1 Monoclonal antibody (66069-1-Ig)

    WB analysis of NIH/3T3 using 66069-1-Ig (same clone as 66069-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using USP1 Monoclonal antibody (66069-1-Ig)

    WB analysis of HSC-T6 using 66069-1-Ig (same clone as 66069-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP1 Monoclonal antibody (66069-1-Ig)

    WB analysis of HeLa using 66069-1-Ig (same clone as 66069-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using USP1 Monoclonal antibody (66069-1-Ig)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using USP1 Monoclonal antibody (66069-1-Ig)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using USP1 Monoclonal antibody (66069-1-Ig)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using USP1 Monoclonal antibody (66069-1-Ig)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

USP1 Monoclonal antibody, PBS Only

USP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66069-1-PBS
Clone No.1C10H6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

1C10H6, Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66069-1-PBS targets USP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP1 fusion protein Ag5904 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 1
Calculated molecular weight 88 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC050525
Gene Symbol USP1
Gene ID (NCBI) 7398
RRIDAB_11043340
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94782
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).