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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP10 Recombinant antibody, PBS Only

USP10 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240583G6

Cat no : 81435-2-PBS

Synonyms

Ubiquitin-specific-processing protease 10, Ubiquitin carboxyl-terminal hydrolase 10, KIAA0190, EC:3.4.19.12, Deubiquitinating enzyme 10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP10 Recombinant antibody (81435-2-RR)

    WB analysis using 81435-2-RR (same clone as 81435-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using USP10 Recombinant antibody (81435-2-RR)

    WB analysis of NIH/3T3 using 81435-2-RR (same clone as 81435-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using USP10 Recombinant antibody (81435-2-RR)

    IF Staining of HepG2 using 81435-2-RR (same clone as 81435-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP10 antibody (81435-2-RR, Clone: 240583G6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81435-2-RR

    Affinity and Kinetic Characterization of 81435-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81435-2-RR against Human USP10 were performed. The affinity constant is 3.99 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 81435-2-PBS is USP10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP10 fusion protein Ag7233 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 10
Calculated molecular weight 87 kDa, 92 kDa
GenBank accession numberBC000263
Gene symbol USP10
Gene ID (NCBI) 9100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.