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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP10 Recombinant antibody

USP10 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240583G6

Cat no : 81435-2-RR

Synonyms

Ubiquitin-specific-processing protease 10, Ubiquitin carboxyl-terminal hydrolase 10, KIAA0190, EC:3.4.19.12, Deubiquitinating enzyme 10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP10 Recombinant antibody (81435-2-RR)

    WB analysis using 81435-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using USP10 Recombinant antibody (81435-2-RR)

    WB analysis of NIH/3T3 using 81435-2-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using USP10 Recombinant antibody (81435-2-RR)

    IF Staining of HepG2 using 81435-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP10 antibody (81435-2-RR, Clone: 240583G6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 81435-2-RR

    Affinity and Kinetic Characterization of 81435-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81435-2-RR against Human USP10 were performed. The affinity constant is 3.99 nM.

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Tested Applications

Positive WB detected inMCF-7 cells, NIH/3T3 cells, HEK-293 cells, A549 cells, LNCaP cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 81435-2-RR is USP10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP10 fusion protein Ag7233 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 10
Calculated molecular weight 87 kDa, 92 kDa
Observed molecular weight 100-130 kDa
GenBank accession numberBC000263
Gene symbol USP10
Gene ID (NCBI) 9100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.

Protocols

Product Specific Protocols
WB protocol for USP10 antibody 81435-2-RRDownload protocol
IF protocol for USP10 antibody 81435-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols