Validation Data Gallery
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Tested Applications
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Ramos cells, Daudi cells |
| Positive IHC detected in | rat testis tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67805-1-Ig targets USP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | USP11 fusion protein Ag19058 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 11 |
| Calculated molecular weight | 963 aa, 110 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC000350 |
| Gene Symbol | USP11 |
| Gene ID (NCBI) | 8237 |
| RRID | AB_2918568 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P51784 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP11 antibody 67805-1-Ig | Download protocol |
| IHC protocol for USP11 antibody 67805-1-Ig | Download protocol |
| IF protocol for USP11 antibody 67805-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |