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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP11 Monoclonal antibody (67805-1-Ig)

    WB analysis using 67805-1-Ig (same clone as 67805-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67805-1-Ig (USP11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USP11 Monoclonal antibody (67805-1-Ig)

    WB analysis of HEK-293 using 67805-1-Ig (same clone as 67805-1-PBS)

    WB result of USP11 antibody (67805-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells. This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using USP11 Monoclonal antibody (67805-1-Ig)

    IHC staining of human stomach cancer using 67805-1-Ig (same clone as 67805-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using USP11 Monoclonal antibody (67805-1-Ig)

    IHC staining of rat testis using 67805-1-Ig (same clone as 67805-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using USP11 Monoclonal antibody (67805-1-Ig)

    IF Staining of HepG2 using 67805-1-Ig (same clone as 67805-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP11 antibody (67805-1-Ig, Clone: 2G6E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

USP11 Monoclonal antibody, PBS Only

USP11 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67805-1-PBS
Clone No.2G6E7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Ubiquitin-specific-processing protease 11, Ubiquitin thiolesterase 11, Ubiquitin thioesterase 11, Ubiquitin carboxyl-terminal hydrolase 11, EC:3.4.19.12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67805-1-PBS targets USP11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP11 fusion protein Ag19058 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 11
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight110 kDa
GenBank accession numberBC000350
Gene Symbol USP11
Gene ID (NCBI) 8237
RRIDAB_2918568
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP51784
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.