Validation Data Gallery
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Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, HepG2 cells, A375 cells, HEK-293 cells, Jurkat cells, MOLT-4 cells |
| Positive IHC detected in | human testis tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
66176-1-Ig targets USP13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | USP13 fusion protein Ag10506 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 13 (isopeptidase T-3) |
| Calculated molecular weight | 863 aa, 97 kDa |
| Observed molecular weight | 97-100 kDa |
| GenBank accession number | BC016146 |
| Gene Symbol | USP13 |
| Gene ID (NCBI) | 8975 |
| RRID | AB_2881571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q92995 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP13 antibody 66176-1-Ig | Download protocol |
| IHC protocol for USP13 antibody 66176-1-Ig | Download protocol |
| IF protocol for USP13 antibody 66176-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Mol Biosci Deubiquitination of MITF-M Regulates Melanocytes Proliferation and Apoptosis.
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Biochim Biophys Acta Mol Basis Dis USP13 inhibition exacerbates mitochondrial dysfunction and acute kidney injury by acting on MCL-1 |