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  • Featured Product
  • KD/KO Validated

USP13 Monoclonal antibody, PBS Only

USP13 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E8C10

Cat no : 66176-1-PBS

Synonyms

Ubiquitin carboxyl-terminal hydrolase 13, ISOT-3, ISOT3, Isopeptidase T-3, EC:3.4.19.12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP13 Monoclonal antibody (66176-1-Ig)

    WB analysis using 66176-1-Ig (same clone as 66176-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP13 Monoclonal antibody (66176-1-Ig)

    WB analysis of HeLa using 66176-1-Ig (same clone as 66176-1-PBS)

    WB result of USP13 antibody (66176-1-Ig, 1:600) with si-Control and si-USP13 transfected HeLa cells. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using USP13 Monoclonal antibody (66176-1-Ig)

    WB analysis of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using USP13 Monoclonal antibody (66176-1-Ig)

    IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using USP13 Monoclonal antibody (66176-1-Ig)

    IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using USP13 Monoclonal antibody (66176-1-Ig)

    IHC staining of human skin cancer using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using USP13 Monoclonal antibody (66176-1-Ig)

    IHC staining of human skin cancer using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using USP13 Monoclonal antibody (66176-1-Ig)

    IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66176-1-Ig (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using USP13 Monoclonal antibody (66176-1-Ig)

    IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP13 antibody (66176-1-Ig, Clone: 6E8C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66176-1-PBS targets USP13 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP13 fusion protein Ag10506 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 13 (isopeptidase T-3)
Calculated molecular weight 863 aa, 97 kDa
Observed molecular weight97-100 kDa
GenBank accession numberBC016146
Gene symbol USP13
Gene ID (NCBI) 8975
RRIDAB_2881571
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.