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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP14 Monoclonal antibody (67746-1-Ig)

    WB analysis using 67746-1-Ig (same clone as 67746-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP14 Monoclonal antibody (67746-1-Ig)

    WB analysis using 67746-1-Ig (same clone as 67746-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using USP14 Monoclonal antibody (67746-1-Ig)

    IF Staining of HepG2 using 67746-1-Ig (same clone as 67746-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP14 antibody (67746-1-Ig, Clone: 3B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

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USP14 Monoclonal antibody, PBS Only

USP14 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67746-1-PBS
Clone No.3B12G3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67746-1-PBS targets USP14 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP14 fusion protein Ag6312 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Calculated molecular weight 54 kDa
Observed molecular weight60 kDa
GenBank accession numberBC003556
Gene Symbol USP14
Gene ID (NCBI) 9097
RRIDAB_2918515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP54578
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.