USP14 Monoclonal antibody
USP14 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig, Rabbit and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
3B12G3
Cat no : 67746-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, U2OS cells, K-562 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
67746-1-Ig targets USP14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
| Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | USP14 fusion protein Ag6312 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC003556 |
| Gene symbol | USP14 |
| Gene ID (NCBI) | 9097 |
| RRID | AB_2918515 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP14 antibody 67746-1-Ig | Download protocol |
| IF protocol for USP14 antibody 67746-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oral Dis USP14 promotes the cancer stem-like cell properties of OSCC via promoting SOX2 deubiquitination | ||
Redox Biol ALDH2 deficiency augments atherosclerosis through the USP14-cGAS-dependent polarization of proinflammatory macrophages | ||
Br J Cancer GSTM3 enhances radiosensitivity of nasopharyngeal carcinoma by promoting radiation-induced ferroptosis through USP14/FASN axis and GPX4 |
