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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated USP14 Monoclonal antibody

USP14 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B12G3

Cat no : CL488-67746

Synonyms

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated USP14 Monoclonal ant (CL488-67746)

    IF Staining of HepG2 using CL488-67746

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 USP14 antibody (CL488-67746, Clone: 3B12G3 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67746 targets USP14 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP14 fusion protein Ag6312 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Calculated molecular weight 54 kDa
Observed molecular weight60 kDa
GenBank accession numberBC003556
Gene symbol USP14
Gene ID (NCBI) 9097
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 USP14 antibody CL488-67746Download protocol
Standard Protocols
Click here to view our Standard Protocols