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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated USP14 Recombinant antibody

USP14 Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

3G12

Cat no : CL750-82128

Synonyms

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated USP14 Recombinant an (CL750-82128)

    WB analysis using CL750-82128

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82128 (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated USP14 Recombinant an (CL750-82128)

    WB analysis using CL750-82128

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82128 (USP14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 750-conjugated USP14 Recombinant an (CL750-82128)

    FC experiment of HepG2 using CL750-82128

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated USP14 Recombinant antibody (CL750-82128, Clone:3G12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, C6 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82128 targets USP14 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP14 fusion protein Ag5979 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Calculated molecular weight 54 kDa
Observed molecular weight60 kDa
GenBank accession numberBC003556
Gene symbol USP14
Gene ID (NCBI) 9097
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 USP14 antibody CL750-82128Download protocol
Standard Protocols
Click here to view our Standard Protocols