Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, C6 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-82128 targets USP14 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | USP14 fusion protein Ag5979 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC003556 |
| Gene Symbol | USP14 |
| Gene ID (NCBI) | 9097 |
| RRID | AB_3673738 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54578 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 USP14 antibody CL750-82128 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |