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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP28 Monoclonal antibody

USP28 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3D1

Cat no : 67814-1-Ig

Synonyms

Ubiquitin thiolesterase 28, Ubiquitin carboxyl-terminal hydrolase 28, KIAA1515, EC:3.4.19.12, Deubiquitinating enzyme 28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP28 Monoclonal antibody (67814-1-Ig)

    WB analysis using 67814-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67814-1-Ig (USP28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using USP28 Monoclonal antibody (67814-1-Ig)

    WB analysis using 67814-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67814-1-Ig (USP28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP28 Monoclonal antibody (67814-1-Ig)

    IF Staining of HeLa using 67814-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using USP28 antibody (67814-1-Ig, Clone: 1E3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inMDA-MB-231 cells, HSC-T6 cells, HSC-T6 cells and NIH/3T3 cells., MDA-MB-468 cells, Hela cells and LNCaP cells.
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67814-1-Ig targets USP28 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP28 fusion protein Ag11805 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 28
Calculated molecular weight583aa,66 kDa; 1077aa,120 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC065928
Gene symbol USP28
Gene ID (NCBI) 57646
RRIDAB_2918577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP28 (Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).

Protocols

Product Specific Protocols
WB protocol for USP28 antibody 67814-1-IgDownload protocol
IF protocol for USP28 antibody 67814-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols