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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP28 Monoclonal antibody (67814-1-Ig)

    WB analysis using 67814-1-Ig (same clone as 67814-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67814-1-Ig (USP28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP28 Monoclonal antibody (67814-1-Ig)

    WB analysis using 67814-1-Ig (same clone as 67814-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67814-1-Ig (USP28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP28 Monoclonal antibody (67814-1-Ig)

    IF Staining of HeLa using 67814-1-Ig (same clone as 67814-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using USP28 antibody (67814-1-Ig, Clone: 1E3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

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USP28 Monoclonal antibody, PBS Only

USP28 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67814-1-PBS
Clone No.1E3D1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Ubiquitin thiolesterase 28, Ubiquitin carboxyl-terminal hydrolase 28, KIAA1515, EC:3.4.19.12, Deubiquitinating enzyme 28

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67814-1-PBS targets USP28 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP28 fusion protein Ag11805 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 28
Calculated molecular weight583aa,66 kDa; 1077aa,120 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC065928
Gene Symbol USP28
Gene ID (NCBI) 57646
RRIDAB_2918577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RU2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

USP28 (Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).