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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP30 Recombinant antibody, PBS Only

USP30 Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240111C6

Cat no : 83240-5-PBS

Synonyms

Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP30 Recombinant antibody (83240-5-RR)

    WB analysis using 83240-5-RR (same clone as 83240-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83240-5-RR (USP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using USP30 Recombinant antibody (83240-5-RR)

    FC experiment of HeLa using 83240-5-RR (same clone as 83240-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug USP30 Recombinant antibody (83240-5-RR, Clone:240111C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83240-5-RR

    Affinity and Kinetic Characterization of 83240-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83240-5-RR against Human USP30 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83240-5-PBS is USP30 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP30 fusion protein Ag7620 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 30
Calculated molecular weight58.5 kDa
GenBank accession numberBC004868
Gene symbol USP30
Gene ID (NCBI) 84749
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.