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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using USP30 Recombinant antibody (83240-5-RR)

    WB analysis of HepG2 using 83240-5-RR (same clone as 83240-5-PBS)

    WB result of USP30 antibody (83240-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP30 transfected HepG2 cells. This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP30 Recombinant antibody (83240-5-RR)

    WB analysis using 83240-5-RR (same clone as 83240-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83240-5-RR (USP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using USP30 Recombinant antibody (83240-5-RR)

    FC experiment of HeLa using 83240-5-RR (same clone as 83240-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug USP30 Recombinant antibody (83240-5-RR, Clone:240111C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83240-5-RR

    Affinity and Kinetic Characterization of 83240-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83240-5-RR against Human USP30 were performed. The affinity constant is below 1 pM.

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  • KD/KO Validated

USP30 Recombinant antibody, PBS Only

USP30 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83240-5-PBS
Clone No.240111C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83240-5-PBS targets USP30 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP30 fusion protein Ag7620 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 30
Calculated molecular weight58.5 kDa
Observed molecular weight 59-67 kDa
GenBank accession numberBC004868
Gene Symbol USP30
Gene ID (NCBI) 84749
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ70CQ3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).