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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP30 Recombinant antibody

USP30 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240111C6

Cat no : 83240-5-RR

Synonyms

Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP30 Recombinant antibody (83240-5-RR)

    WB analysis using 83240-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83240-5-RR (USP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using USP30 Recombinant antibody (83240-5-RR)

    FC experiment of HeLa using 83240-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug USP30 Recombinant antibody (83240-5-RR, Clone:240111C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83240-5-RR

    Affinity and Kinetic Characterization of 83240-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83240-5-RR against Human USP30 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inJurkat cells, A549 cells, HEK-293 cells, SH-SY5Y cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83240-5-RR is USP30 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP30 fusion protein Ag7620 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 30
Calculated molecular weight58.5 kDa
Observed molecular weight 59-67 kDa
GenBank accession numberBC004868
Gene symbol USP30
Gene ID (NCBI) 84749
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).

Protocols

Product Specific Protocols
WB protocol for USP30 antibody 83240-5-RRDownload protocol
FC protocol for USP30 antibody 83240-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols