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USP5 Monoclonal antibody

Name: USP5 Monoclonal antibody
Brand: Proteintech
SKU: 66213-1-Ig

USP5 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

3F12C10

Review Product

Cat no : 66213-1-Ig

Synonyms

ISOT 1, ISOT, Isopeptidase T, EC:3.4.19.12, Deubiquitinating enzyme 5

Validation Data Gallery

Filter:

AllWBIF/ICC

WB analysis using 66213-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis of A549 using 66213-1-Ig
WB result of USP5 antibody (66213-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.
WB analysis of Jurkat using 66213-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66213-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66213-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 66213-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IF Staining of A549 using 66213-1-Ig
Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP5 antibody (66213-1-Ig, Clone: 3F12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Cat No. 66213-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, A549 cells, LNCaP cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

66213-1-Ig targets USP5 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	USP5 fusion protein Ag7575 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 5 (isopeptidase T)
Calculated molecular weight	96 kDa
Observed molecular weight	95-105 kDa
GenBank accession number	BC005139
Gene symbol	USP5
Gene ID (NCBI)	8078
RRID	AB_2881604
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

Protocols

Product Specific Protocols
WB protocol for USP5 antibody 66213-1-Ig	Download protocol
IF protocol for USP5 antibody 66213-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy Authors - Xiangling Xiao View Article
human,mouse	IF,CoIP	Cell Commun Signal Deubiquitinase USP5 regulates RIPK1 driven pyroptosis in response to myocardial ischemic reperfusion injury Authors - Wenjing Sun View Article
rat	WB	Sci Rep Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies. Authors - Katrin Juenemann View Article

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研究分野

Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP5 Monoclonal antibody

USP5 Monoclonal Antibody for WB, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 66213-1-Ig

WB analysis of A549 using 66213-1-Ig

WB analysis of Jurkat using 66213-1-Ig

WB analysis of HeLa using 66213-1-Ig

WB analysis of HeLa using 66213-1-Ig

WB analysis of HEK-293 using 66213-1-Ig

IF Staining of A549 using 66213-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy

Deubiquitinase USP5 regulates RIPK1 driven pyroptosis in response to myocardial ischemic reperfusion injury

Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies.