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  • Featured Product
  • KD/KO Validated

USP5 Monoclonal antibody, PBS Only

USP5 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F12C10

Cat no : 66213-1-PBS

Synonyms

Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis using 66213-1-Ig (same clone as 66213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

    WB result of USP5 antibody (66213-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of Jurkat using 66213-1-Ig (same clone as 66213-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of HeLa using 66213-1-Ig (same clone as 66213-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of HeLa using 66213-1-Ig (same clone as 66213-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of HEK-293 using 66213-1-Ig (same clone as 66213-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using USP5 Monoclonal antibody (66213-1-Ig)

    IF Staining of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP5 antibody (66213-1-Ig, Clone: 3F12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66213-1-PBS targets USP5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP5 fusion protein Ag7575 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 5 (isopeptidase T)
Calculated molecular weight 96 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC005139
Gene symbol USP5
Gene ID (NCBI) 8078
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.