• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using USP9X Recombinant antibody (81892-1-RR)

    WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    WB result of USP9X antibody (81892-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP9X transfected HEK-293 cells. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using USP9X Recombinant antibody (81892-1-RR)

    WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 81892-1-RR (USP9X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using USP9X Recombinant antibody (81892-1-RR)

    IP experiment of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    IP result of anti-USP9X (IP:81892-1-RR, 4ug; Detection:81892-1-RR 1:3000) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using USP9X Recombinant antibody (81892-1-RR)

    IF Staining of mouse testis using 81892-1-RR (same clone as 81892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP9X Recombinant antibody (81892-1-RR)

    IF Staining of HeLa using 81892-1-RR (same clone as 81892-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

USP9X Recombinant antibody, PBS Only

USP9X Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 81892-1-PBS
Clone No.1B23

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

FAM, FAF, EC:3.4.19.12, DFFRX, Deubiquitinating enzyme FAF-X

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81892-1-PBS targets USP9X in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 9, X-linked
Calculated molecular weight 292 kDa
Observed molecular weight260-290 kDa
GenBank accession numberNM_001039590
Gene Symbol USP9X
Gene ID (NCBI) 8239
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93008
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.