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USP9X Recombinant antibody

USP9X Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B23

Cat no : 81892-1-RR

Synonyms

Deubiquitinating enzyme FAF X, DFFRX, FAF, FAM, Fat facets in mammals, hFAM, Ubiquitin thiolesterase FAF X, USP9, USP9X

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using USP9X Recombinant antibody (81892-1-RR)

    WB analysis of HEK-293 using 81892-1-RR

    WB result of USP9X antibody (81892-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP9X transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using USP9X Recombinant antibody (81892-1-RR)

    WB analysis of HEK-293 using 81892-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 81892-1-RR (USP9X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using USP9X Recombinant antibody (81892-1-RR)

    IP experiment of HEK-293 using 81892-1-RR

    IP result of anti-USP9X (IP:81892-1-RR, 4ug; Detection:81892-1-RR 1:3000) with HEK-293 cells lysate 1640 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP9X Recombinant antibody (81892-1-RR)

    IF Staining of HeLa using 81892-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, A549 cells, K-562 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81892-1-RR targets USP9X in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 9, X-linked
Calculated molecular weight 292 kDa
Observed molecular weight260-290 kDa
GenBank accession numberNM_001039590
Gene symbol USP9X
Gene ID (NCBI) 8239
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.

Protocols

Product Specific Protocols
WB protocol for USP9X antibody 81892-1-RRDownload protocol
IF protocol for USP9X antibody 81892-1-RRDownload protocol
IP protocol for USP9X antibody 81892-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols