CoraLite® Plus 488-conjugated VAMP2 Monoclonal antibody
VAMP2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG3
Reactivity
Human, Mouse, Rat, Pig, Rabbit
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G7E8
Cat no : CL488-67822
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67822 targets VAMP2 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
| Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | VAMP2 fusion protein Ag17908 相同性解析による交差性が予測される生物種 |
| Full Name | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| Calculated molecular weight | 13 kDa |
| Observed molecular weight | 19 kDa |
| GenBank accession number | BC002737 |
| Gene symbol | VAMP2 |
| Gene ID (NCBI) | 6844 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VAMP2 antibody CL488-67822 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
