Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
67219-1-Ig targets VAMP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse samples.
| Tested Reactivity | Human, Pig, Rat, Mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | VAMP4 fusion protein Ag27854 相同性解析による交差性が予測される生物種 |
| Full Name | vesicle-associated membrane protein 4 |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC007019 |
| Gene Symbol | VAMP4 |
| Gene ID (NCBI) | 8674 |
| RRID | AB_2882510 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VAMP4 antibody 67219-1-Ig | Download protocol |
| IHC protocol for VAMP4 antibody 67219-1-Ig | Download protocol |
| IF protocol for VAMP4 antibody 67219-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
eNeuro ADP ribosylation factor 4 (Arf4) regulates radial migration through N-cadherin trafficking during cerebral cortical development |