Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67219 targets VAMP4 in IF/ICC applications and shows reactivity with Human, Pig, Rat, Mouse samples.
| Tested Reactivity | Human, Pig, Rat, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | VAMP4 fusion protein Ag27854 相同性解析による交差性が予測される生物種 |
| Full Name | vesicle-associated membrane protein 4 |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC007019 |
| Gene Symbol | VAMP4 |
| Gene ID (NCBI) | 8674 |
| RRID | AB_2919438 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75379 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VAMP4 antibody CL488-67219 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |