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  • Featured Product
  • KD/KO Validated

VAPB Polyclonal antibody

VAPB Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14477-1-AP

Synonyms

VAMP C, VAMP B/VAMP C, VAMP B, VAMP associated protein B/C, UNQ484/PRO983

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using VAPB Polyclonal antibody (14477-1-AP)

    WB analysis of HepG2 using 14477-1-AP

    WB result of VAPB antibody (14477-1-AP, 1:5000) with si-control and si-VAPB transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using VAPB Polyclonal antibody (14477-1-AP)

    WB analysis using 14477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using VAPB Polyclonal antibody (14477-1-AP)

    WB analysis of rat brain using 14477-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using VAPB Polyclonal antibody (14477-1-AP)

    WB analysis of mouse skeletal muscle using 14477-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using VAPB Polyclonal antibody (14477-1-AP)

    WB analysis of HepG2 using 14477-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using VAPB Polyclonal antibody (14477-1-AP)

    IP experiment of HeLa using 14477-1-AP

    IP result of anti-VAPB (IP:14477-1-AP, 3ug; Detection:14477-1-AP 1:800) with HeLa cells lysate 1840ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of human breast cancer using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of human breast cancer using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of human pancreas cancer using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of human pancreas cancer using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of mouse liver using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of mouse liver using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of human pancreas cancer using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of human pancreas cancer using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of human breast cancer using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VAPB Polyclonal antibody (14477-1-AP)

    IHC staining of human breast cancer using 14477-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VAPB Polyclonal antibody (14477-1-AP)

    IF Staining of HeLa using 14477-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAPB antibody (14477-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VAPB Polyclonal antibody (14477-1-AP)

    IF Staining of HepG2 using 14477-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VAPB antibody (14477-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using VAPB Polyclonal antibody (14477-1-AP)

    FC experiment of HepG2 using 14477-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug VAPB Polyclonal antibody (14477-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, HepG2 cells, rat brain tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, mouse liver tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14477-1-AP targets VAPB in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAPB fusion protein Ag5857 相同性解析による交差性が予測される生物種
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001712
Gene symbol VAPB
Gene ID (NCBI) 9217
RRIDAB_2288297
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late-onset spinal muscular atrophy.

Protocols

Product Specific Protocols
WB protocol for VAPB antibody 14477-1-APDownload protocol
IHC protocol for VAPB antibody 14477-1-APDownload protocol
IF protocol for VAPB antibody 14477-1-APDownload protocol
IP protocol for VAPB antibody 14477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

A High-Density Human Mitochondrial Proximity Interaction Network.

Authors - Hana Antonicka
View Article
monkeyWB

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
View Article
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
View Article
monkeyWB

Mol Cell

MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.

Authors - Christophe A C Freyre
View Article
humanWB

J Clin Invest

SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.

Authors - Museer A Lone
View Article
humanIF

Cancer Res

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

Authors - Bingteng Xie
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Wei (Verified Customer) (09-22-2020)

The antibody have predicted band on western blot.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse heart