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Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 555-conjugated VAPB Polyclonal anti (CL555-14477)

    FC experiment of Jurkat using CL555-14477

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human VAPB (CL555-14477) (red), or 0.2 ug Rabbit IgG CL555-30000(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 555-conjugated VAPB Polyclonal anti (CL555-14477)

    FC experiment of HepG2 using CL555-14477

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 555-conjugated VAPB Polyclonal antibody (CL555-14477)(red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated VAPB Polyclonal antibody

VAPB Polyclonal Antibody for FC (Intra)
Cat No. CL555-14477

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

VAMP C, VAMP B/VAMP C, VAMP B, VAMP associated protein B/C, UNQ484/PRO983

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 555
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inJurkat cells, HepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-14477 targets VAPB in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAPB fusion protein Ag5857 相同性解析による交差性が予測される生物種
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001712
Gene Symbol VAPB
Gene ID (NCBI) 9217
RRIDAB_3084528
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95292
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 VAPB antibody CL555-14477Download protocol
Standard Protocols
Click here to view our Standard Protocols