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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using VAPB Monoclonal antibody (66191-1-Ig)

    WB analysis of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

    WB result of VAPB antibody (66191-1-Ig, 1:5000) with si-control and si-VAPB transfected HepG2 cells. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VAPB Monoclonal antibody (66191-1-Ig)

    WB analysis using 66191-1-Ig (same clone as 66191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VAPB Monoclonal antibody (66191-1-Ig)

    WB analysis using 66191-1-Ig (same clone as 66191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VAPB Monoclonal antibody (66191-1-Ig)

    IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VAPB Monoclonal antibody (66191-1-Ig)

    IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VAPB Monoclonal antibody (66191-1-Ig)

    IHC staining of mouse kidney using 66191-1-Ig (same clone as 66191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66191-1-Ig (VAPB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VAPB Monoclonal antibody (66191-1-Ig)

    IF Staining of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPB antibody (66191-1-Ig, Clone: 4F6A6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VAPB Monoclonal antibody (66191-1-Ig)

    IF Staining of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66191-1-Ig (VAPB antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

VAPB Monoclonal antibody, PBS Only

VAPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66191-1-PBS
Clone No.4F6A6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66191-1-PBS targets VAPB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VAPB fusion protein Ag6313 相同性解析による交差性が予測される生物種
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Calculated molecular weight 27 kDa
Observed molecular weight30-32 kDa
GenBank accession numberBC001712
Gene Symbol VAPB
Gene ID (NCBI) 9217
RRIDAB_2881586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95292
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.