Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, LNCaP cells, MCF-7 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 19 publications below |
| IF | See 11 publications below |
| CoIP | See 3 publications below |
Product Information
66191-1-Ig targets VAPB in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, monkey, yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6313 Product name: Recombinant human VAPB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 31-180 aa of BC001712 Sequence: KLGNPTDRNVCFKVKTTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEV 相同性解析による交差性が予測される生物種 |
| Full Name | VAMP (vesicle-associated membrane protein)-associated protein B and C |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 30-32 kDa |
| GenBank accession number | BC001712 |
| Gene Symbol | VAPB |
| Gene ID (NCBI) | 9217 |
| RRID | AB_2881586 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95292 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VAPB antibody 66191-1-Ig | Download protocol |
| IHC protocol for VAPB antibody 66191-1-Ig | Download protocol |
| WB protocol for VAPB antibody 66191-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites
| ||
Autophagy The ER protein CANX (calnexin)-mediated autophagy protects against alzheimer disease | ||
Nat Commun Restructured membrane contacts rewire organelles for human cytomegalovirus infection | ||
Nat Commun ER-mitochondria contacts mediate lipid radical transfer via RMDN3/PTPIP51 phosphorylation to reduce mitochondrial oxidative stress | ||
Nat Commun RHOA regulates mitochondria-ER contact sites through modulation of the VAPB/PTPIP51 tether. |