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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated VAPB Monoclonal antibody

VAPB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4F6A6

Cat no : CL488-66191

Synonyms

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated VAPB Monoclonal anti (CL488-66191)

    IF Staining of HepG2 using CL488-66191

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 VAPB antibody (CL488-66191, Clone: 4F6A6 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66191 targets VAPB in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VAPB fusion protein Ag6313 相同性解析による交差性が予測される生物種
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001712
Gene symbol VAPB
Gene ID (NCBI) 9217
RRIDAB_2919296
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VAPB antibody CL488-66191Download protocol
Standard Protocols
Click here to view our Standard Protocols