VCP Monoclonal antibody, PBS Only

U2OS (WT and VCP KD) lysates prepared with RIPA buffer, 10μg protein loaded. 60316-1-Ig incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

WB result of VCP antibody (60316-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells. This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60316-1-Ig (VCP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (60316-1-Ig, Clone: 2A4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60316-1-Ig(VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

1X10^6 HL-60 cells were intracellularly stained with 0.4 ug Anti-Human VCP (60316-1-Ig, Clone:2A4B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.