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VCP 抗体 2 文献

Mouse Monoclonal| カタログ番号: 60316-1-Ig |クローン番号: 2A4B10

注目製品 KD/KO 検証済

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適用アプリケーション

アプリケーション:
WB, IHC, IF, ELISA
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Images See all

VCP Antibody WB HeLa cells 60316-1-Ig

WB result of VCP antibody (60316-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells.

VCP Antibody WB RAW 264.7 cells 60316-1-Ig

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

VCP Antibody IHC human gliomas tissue 60316-1-Ig

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 10x lens).

VCP Antibody IHC human gliomas tissue 60316-1-Ig

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 40x lens).

VCP Antibody IF RAW 264.7 cells 60316-1-Ig

Immunofluorescent analysis of RAW 264.7 cells using 60316-1-Ig (VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

種特異性:
human, mouse
WB ポジティブ:
RAW 264.7 cells, HeLa cells, SH-SY5Y cells
IHC ポジティブ:
human gliomas tissue
IF ポジティブ:
RAW 264.7 cells, SH-SY5Y cells
推奨希釈倍率:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein G purification
アイソタイプ:
IgG1
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
VCP fusion protein Ag1002
正式名:
valosin-containing protein
計算分子量:
89 kDa
検出分子量:
89 kDa
GenBank accession number:
BC007562
Gene ID (NCBI):
7415
遺伝子シンボル:
VCP
別名:
15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

背景説明

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).


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