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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated VCP Monoclonal antibody

VCP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A4B10

Cat no : CL594-60316

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated VCP Monoclonal antibody (CL594-60316)

    IF Staining of HeLa using CL594-60316

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60316 (VCP antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated VCP Monoclonal antibody (CL594-60316)

    IF Staining of HeLa using CL594-60316

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 VCP antibody (CL594-60316, Clone: 2A4B10 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60316 targets VCP in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VCP fusion protein Ag1002 相同性解析による交差性が予測される生物種
Full Name valosin-containing protein
Calculated molecular weight 89 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC007562
Gene symbol VCP
Gene ID (NCBI) 7415
RRIDAB_2883462
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Protocols

Product Specific Protocols
IF protocol for CL594 VCP antibody CL594-60316Download protocol
Standard Protocols
Click here to view our Standard Protocols